Two classes of spontaneous miniature excitatory junction potentials and one synaptic vesicle class are present in the ray electrocyte.

Abstract

Cross sections (1-2 mm thick) of the ray (Raja) tail were secured to a dish and immersed in elasmobranch saline. Spontaneous miniature excitatory junction potentials (MEJPs) were recorded by advancing a 50 k omega, KCl filled electrode into the electric organ (20 microV peak-to-peak baseline noise). Data were filmed, and/or recorded on magnetic tape for computer analyses. Intracellularly recorded MEJP amplitude histograms showed a peak at 60 microV and had a right-hand skew with MEJPs up to 0.5 mV. The small peak amplitude and the skewed amplitude distribution of intracellularly recorded MEJPs result from the relatively low input resistance and the short space constant of the electrocyte coupled with the dispersed synapses on the electrocyte. At 23 degrees C the intracellularly recorded MEJP frequency ranged from 1-10 MEJPs/s. The MEJPs became larger and became focally recorded as the electrode was advanced against the intracellular surface of the innervated membrane of the electrocyte. Focal extracellular MEJPs (reversed polarity) were also recorded with the electrode positioned against the outside surface of the innervated side of the electrocyte. The frequency of focally recorded intracellular MEJPs was increased (up to 40/s) when the electrode was pushed against the membrane. Focal MEJP frequencies decreased to a few/min within 5-10 min but the mean amplitude of 3-5 mV remained constant. Decreases in amplitude and frequency in focally recorded intracellular MEJPs are attributed to changes in electrode pressure against the membrane. Amplitude histograms were constructed from focally recorded intracellular or extracellular MEJPs which showed the same time characteristics. The focal MEJP amplitude histograms have two distinct classes, each forming a bell-shaped distribution. It is concluded that both classes are generated at the electrode tip. The smaller class of MEJPs has a mean 1/10th that of the larger class and composes about 2% of the MEJPs. The small class is analogous to the sub-MEPP class found in the frog sartorius (Kriebel and Gross 1974) and mouse diaphragm (Kriebel et al. 1976, 1982). Distributions of synaptic vesicle diameters are slightly log normal (right hand skew) such that the mean diameter (57 nm) is slightly larger than the modal value (52 nm). Vesicles touching the membrane were of the same size and diameter distribution as the entire vesicle population. The profiles of the distributions are smooth and suggest only 1 class of synaptic vesicle based on diameter.