Simultaneous intracellular and focal extracellular recording of junction potentials and currents, and the time course of quantal transmitter action in rodent vas deferens

Abstract

Simultaneous recordings were made of spontaneous excitatory junction potentials and the underlying spontaneous excitatory junction currents in guinea-pig and mouse vas deferens using adjacent intracellular and focal extracellular electrodes. Concurrent spontaneous excitatory junction potentials and spontaneous excitatory junction currents were observed in a small proportion of smooth muscle cells penetrated intracellularly within 50–200 μ of the extracellular electrode. These simultaneous events had identical variations in time course, indicating that they were caused by the same transmitter release event. Their amplitudes were not related. Concurrent spontaneous excitatory junction potentials and currents had identical durations, rise times and time constants of decay, showing that the spontaneous excitatory junction potential reflects the time course of quantal transmitter action. In contrast, spontaneous “discrete events” obtained by differentiating the rising phases of spontaneous excitatory junction potentials with respect to time were brief compared with the underlying currents. Excitatory junction potentials evoked by electrical stimulation of the hypogastric nerve were prolonged compared to the underlying excitatory junction currents. The peaks in the first time differential of individual excitatory junction potentials (evoked discrete events) were brief compared to corresponding excitatory junction currents. It is concluded that at the neuroeffector junction of the rodent vas deferens the membrane potential response to a quantum of spontaneously released transmitter is a good estimate of the duration of transmitter action, in accordance with some of the predictions for three-dimensional syncytial tissues. The first time differential of the membrane potential, the “discrete event”, does not reflect the time course of spontaneous or evoked quantal transmitter action in these syncytial tissues.