Abstract
Tussilagone (TSL) is a sesquiterpenoid isolated from Tussilago farfara, which has been used as a traditional medicine for the treatment of asthma and bronchitis. It also takes part in the anti-inflammatory and antioxidant effects, but its role in angiogenesis is unknown. Angiogenesis is a cancer feature that is essential for supplying oxygen and nutrients to all proliferating tumor cells. Here, we demonstrated that TSL significantly inhibited the proliferation, migration, invasion, and tube formation of primary human umbilical vascular endothelial cell (HUVEC) in vitro. Also, TSL inhibited vascular endothelial growth factor (VEGF)-induced angiogenesis revealed by Matrigel plug assay in vivo. At present, we observed that TSL inhibited the activity of VEGFR2 signal pathway induced by VEGF. These findings suggested that TSL may serve as a potential therapeutic target in the angiogenesis.
Highlights
Angiogenesis means new blood vessels formation, a process from preexisting vessels, including migration, proliferation, and formation of capillary tubes steps in endothelial cells
DNA synthesis was detected by EdU kit, the results indicated that the cell proliferation ability was apparently improved under the stimulation of vascular endothelial growth factor (VEGF), which was inhibited by TSL
Cabozanitib (XL184), which is an effective inhibitor of VEGFR2, suppressed cell proliferation significantly at a concentration of 5 μM, but the effect was weaker than 30 μM in the TSL group (Supplement Figure 1)
Summary
Angiogenesis means new blood vessels formation, a process from preexisting vessels, including migration, proliferation, and formation of capillary tubes steps in endothelial cells. It is crucial for organ development and wound healing under physiological condition (Kerbel and Folkman, 2002; Jubb et al, 2006; Kunimasa et al, 2011; Lam et al, 2012). It takes part in a series of diseases, such as diabetic retinopathy, peripheral vascular disease, endometriosis, tissue regeneration, atherosclerosis, obesity, rheumatoid arthritis, and cancer (Piao et al, 2018).
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