Abstract
The objective of this study is to evaluate the presence of flavonoids in citrus fıber using reverse-phase high-pressure liquid chromatography (RP-HPLC). In this study, we also investigate the effect of regular and hot-washing preparations on total polyphenol content (TPC), total flavonoid content (TFC), oxygen radical absorbance capacity (ORAC) and total carotenoid content (TCC) of citrus fiber. The HPLC method used provided fast results and good separation for polymethoxyflavones and flavonols. Quercetin and kaempferol (flavonols), and sinensetin, nobiletin, heptamethoxyflavone, and tangeretin (polymethoxyflavones) were identified. Nobiletin had the highest concentration with 3.33 mg/g dry weight followed by sinensetin (1.96 mg/g dry weight) and heptamethoxyflavone (1.24 mg/g dry weight), respectively. The TFC and TCC of citrus fiber were unaffected (P >0.05) by the citrus fiber production procedure; however, the TPC and the ORAC values of citrus fiber prepared using hot-washing were significantly lower (P < 0.05).
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
