Abstract

ABSTRACT A simple, fast, sensitive and accurate turbidimetric assay of lipids in the milled seeds was developed. The procedure is based on the extraction of lipids into acetone followed by their turbidimetric reaction with a 1.5% solution of sulfosalicylic acid and turbidimetric measurements. The spectral measurement was made at 440 nm against a blank of the reagents. A standard curve was constructed using raw fat extracted from the species being analyzed. For analysis of a large number of solvents, the standardization of the turbidimetric method may be accomplished using Soxhlet values for a few representative samples. A negative relationship between the hardness of maize particles and their content of raw fat was observed.

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