Abstract
Interferon-Gamma (IFN-ɣ), a measurable biomarker is known to be related to Tuberculosis which is one of the major sanitation issues in the world. It is also a crucial indicator of the immune response to many infections and diseases. Measuring the concentration of IFN-γ can assist in disease diagnosis. Therefore, we have developed a cost-efficient, disposable, and highly sensitive paper-based electrochemical biosensor with probing aptamer immobilization that can detect IFN-ɣ with high sensitivity and selectivity. WS2 nanotubes (NTs) were modified on the nanocomposite conductive paper electrode to provide a large surface area, facilitating the efficient recognition of aptamer with IFN-ɣ, and yielded a high resistance to adsorption of non-targeted molecules. The step-wise modification of the sensing surface was characterized and then the electrode was examined by using differential pulse voltammetry (DPV) in different concentrations of IFN-ɣ samples. The peak current generated by the proposed sensor demonstrated a linear range from 3.125 to 100 pg mL−1, and the detection limit for IFN-ɣ was determined to be 1.13 pg mL−1. Moreover, this novel WS2 NTs enhanced nanocomposite conductive paper-based electrochemical biosensor was qualified to successfully measure IFN-ɣ in human serum samples, demonstrating its potential application as an efficient tool for Tuberculosis diagnosis.
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