Tunable viscoelastic size-based particle separation in straight microchannels with triangular cross-sections

Abstract

In recent years, viscoelastic particle manipulation within microfluidic systems has received much attention due to the ease with which micron-sized objects may be maneuvered and isolated on the basis of size. While several factors, including both fluid and particle properties, regulate the precise locations and trajectories of micron-sized species flowing along a microchannel, the role of channel cross-section shape is critical, since it directly influences the fluid velocity profile and thus the direction and magnitude of hydrodynamic forces. It is therefore surprising that this parameter has not been comprehensively investigated for cell-based separations, especially since most viscoelastic microfluidic systems are only able to efficiently sperate cells over limited size ranges. To address this shortcoming, we present a viscoelastic microfluidic system integrating a triangular cross-section microchannel, for efficient and tunable size-based separations of micron-sized species. We find that particle focusing patterns can be controlled by simple variation of volumetric flow rates, which allows for the efficient separation of particles and cells of variable size. To showcase the efficacy of the approach, we present a size-based separation of various blood components, including white blood cells, platelets, and rare cells. By quantifying the number of particles collected at the outlets, we achieve recovery efficiencies of over 98%.