Tumour rejection properties of solubilised TSTA from an SV40-induced neoplasm

Abstract

THE neoplasm mKSA, an SV40-induced sarcoma in BALB/c strain mice has strong, SV40-specific tumour rejection antigens (TSTA). This parental line is also very oncogenic with a tumour dose 50 (TD50) of 102 cells. A subline, passaged in monolayer tissue culture (mKSA-TC), has a much reduced tumour-inducing capacity (TD50 = 2 × 106–2 × 107, cells). Of these mKSA-TC cells 1 × 105 will immunise syngeneic hosts against a challenge of 1 × 106 mKSA cells carried in an ascitic form (mKSA-ASC); immunisation against other SV40-induced BALB/c sarcomas (for example, SVA31C14 and E4) has also been achieved, but not against Adj-PC-5 (ref. 1) nor against Meth A (ref. 2), both neoplasms carrying specific TSTAs and syngeneic with BALB/c but induced by chemical carcinogens.