Abstract
Objective: to compare the morphological and functional properties of mesenchymal stem cells from mammary tissues and chemically-induced mammary tumor tissues. material and methods. The study included 25 female Wistar rats. In 20 rats, mammary carcinoma was induced by intramammary injection of N-methyl-N-nitrosourea after estrus synchronization with chorionic gonadotropin. The control group consisted of 5 rats. Mammary carcinoma was verified histologically and immunohistochemically. To examine, whether the cells isolated from normal tissue and tumor tissue belonged to mesenchymal stem cells, FACS Canto II flow cytofluorometer was used. The functional properties of mesenchymal stem cells were evaluated in MTT assay by the level of nitric oxide production in normal and by hydrogen peroxide-induced hypoxia. The levels of prolactin, luteinizing hormone and estradiol E2 in urine were studied using solid-phase enzyme-linked immunosorbent assay. results. Chemically-induced mammary tumor according to histological and immunohistochemical studies corresponded to luminal B type breast cancer in humans. In rats that developed mammary tumors, the urine prolactin levels after synchronization of estrus were increased. In rats that did not develop tumors, the levels of prolactin and luteinizing hormone were decreased, but the levels of estradiol E2 were increased. More mesenchymal stem cells with CD45-/CD90+phenotype were obtained from the breast tumor tissue. Mesenchymal stem cells from tumor tissue showed increased proliferative potential and were more resistant to hypoxia. conclusion. Tumor- associated mesenchymal stem cells having high proliferative potential and resistance to hypoxia were obtained from chemically-induced mammary tumor tissue. Morphologic and functional differences in mesenchymal stem cells obtained from mammary breast tissue and tumor tissue require further studies.
Highlights
Цель исследования – изучение морфофунк‐ циональных свойств мезенхимных стволовых/стромальных клеток (МСК) крыс-самок Wistar, выделенных из ткани химически индуцированной опухоли молочной железы, контралатеральной молочной железы и ткани молочной железы ин‐ тактных животных
Whether the cells isolated from normal tissue and tumor tissue belonged to mesenchymal stem cells, FACS Canto II flow cytofluorometer was used
The functional properties of mesenchymal stem cells were evaluated in MTT assay by the level of nitric oxide production in normal and by hydrogen peroxide-induced hypoxia
Summary
Цель исследования – сравнить морфофункциональные свойства мезенхимных стволовых клеток из тканей молочной железы и ткани химически-индуцированной опухоли молочной железы. Уровни пролактина в моче после синхронизации эструса у животных с развившейся опухолью были повышены, а у животных с неразвившейся опухолью молочной железы снижены уровни пролактина и лютеинизирующего гормона, но повышены уровни эстрадиола Е2. Из ткани опухоли молочной железы получено большее количество мезенхимных стволовых клеток с фенотипом CD45-/CD90+. Мезенхимные стволовые клетки из ткани опухоли проявляли повышенный пролиферативный потенциал и были более устойчивы к окислительному стрессу. Из ткани химически индуцированной опухоли молочной железы получены опухоль-ассоциированные мезенхимные стволовые клетки, которые имеют высокий пролиферативный потенциал и устойчивы к окислительному стрессу. ЛАБОРАТОРНЫЕ И ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ морфофункциональные различия мезенхимных стволовых клеток из тканей молочных желез и ткани опухоли молочной железы требуют дальнейшего исследования. Ключевые слова: опухоль молочной железы, мезенхимные стволовые клетки, пролиферация, оксид азота, половые гормоны, моча.
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