Abstract
Summary We have studied possible changes in tRNA level during the differentiation of normal hepatic tissue into an ascitic hepatoma, by comparing the specific activities of rat liver and Zajdela hepatoma tRNAs. The standard aminoacylation conditions found for one aminoacid could not be used for the others in charging the tRNAs obtained from the two sources, since it was found that an excess of enzyme in the reaction mixture or an extended incubation time led to a decrease of the acylation level for several tRNA's. Therefore for each aminoacid a study was performed to determine the optimal conditions for aminocylation. For some aminoacids (val, arg, pro) differences were observed in the acylation levels between liver tRNA and hepatoma tRNA, when the same enzymic extrat was used. Differences in the aminoacylation levels (for instance for tRNAgly, tRNAtyr,...) were also revealed when both enzymic preparations (from liver and hepatoma) were used on the same tRNA from liver or hepatoma.
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