Abstract
Tubeimoside-1 (TBMS1) is a triterpenoid saponin with potent anticancer properties. In this study, for the first, we examined the anti-proliferative effects of TBMS1 in human bladder cancer T24 cells and its ability to induce apoptosis and cell cycle arrest. Our results demonstrated that TBMS1 decreased the cell viability of bladder cancer T24 cells in a dose-dependent manner. Flow cytometric analysis showed that TBMS1 significantly triggered apoptosis in T24 cells and arrested cell cycle at G2/M phase in a dose-dependent manner. Further characterization demonstrated that TBMS1-induced apoptosis is associated with dissipation in mitochondrial membrane potential (Δ Ψ m ), down-regulation of Bcl-2, and up-regulation of Bax and p21 in TBMS1-treated T24 cells. These in vitro results suggested that TBMS1 is an effective anti-bladder cancer natural compound that worth further mechanistic and therapeutic studies in human bladder cancer. DOI: http://dx.doi.org/10.3329/bjp.v9i4.19989
Highlights
Human bladder cancer is a potentially lethal and aggressive tumor has continuously increasing incidence all over the world (Ploeg et al, 2009)
Human bladder cancer T24 cells were treated with different concentrations of TBMS1 (3, 6, 12.5, 25, 50 and 100 μM) for 24 hours
We found that TBMS1 exhibited cytotoxic effects on the propagation of T24 cells
Summary
Human bladder cancer is a potentially lethal and aggressive tumor has continuously increasing incidence all over the world (Ploeg et al, 2009). To probe the changes in ΔΨm, T24 cells were stained with rhodamine-123 (1 μM) after treatment with 10 and 20 μM of TBMS1 for 24 hours with control group. Human bladder cancer T24 cells were treated with different concentrations of TBMS1 (3, 6, 12.5, 25, 50 and 100 μM) for 24 hours.
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