Jaceosidin inhibits proliferation of human bladder cancer T24 cells through induction of cell cycle arrest and apoptosis

Yong Li,Yan Tan

doi:10.3329/bjp.v8i3.15620

Abstract

Jaceosidin, isolated from Artemesia argyi , has been shown to possess promising anticancer potential against various cancer cells. However, its effect against bladder cancer cells remained unknown. In this study, for the first time, we investigated the effects of jaceosidin on cell proliferation, cell cycle, and apoptosis in bladder cancer T24 cells by using MTT assay and flow cytometric analysis. The results revealed that jaceosidin decreased the cell viability of bladder cancer T24 cells in a dose- and time-dependent manner. Flow cytometric analysis demonstrated that jaceosidin significantly triggered apoptosis in T24 cells and arrested cell cycle at G2/M phase in a time-dependent manner. Further characterization showed that jaceosidin-induced apoptosis is associated with dissipation in mitochondrial membrane potential (ΔΨ m ), up-regulation of Bax and down-regulation of Bcl-2 in jaceosidin-treated T24 cells. These in vitro results suggested that jaceosidin should be further examined for in vivo activity and molecular mechanism in human bladder cancer. DOI: http://dx.doi.org/10.3329/bjp.v8i3.15620 Bangladesh Journal of Pharmacology Vol.8(3) 2013 349-356

Highlights

Natural products have become one of the most imperative sources of prospective anticancer agents and several compounds, which were originally isolated from plants such as paclitaxel, camptothecin, vinca alkaloids, and etoposide have potential applications in cancer chemotherapy, plants are considered as one of the most important sources for the development of novel anti-cancer drugs (Amin et al, 2009; Cragg and Newman, 2005)
We found that jaceosidin effectively exhibited cytotoxic effects on the growth of T24 cells
The effects of jaceosidin were examined on the growth of T24 human bladder cancer cells by quantifying the viable cells using MTT assay

Summary

Introduction

Natural products have become one of the most imperative sources of prospective anticancer agents and several compounds, which were originally isolated from plants such as paclitaxel, camptothecin, vinca alkaloids, and etoposide have potential applications in cancer chemotherapy, plants are considered as one of the most important sources for the development of novel anti-cancer drugs (Amin et al, 2009; Cragg and Newman, 2005). Artemisia plants possess a variety of biological activities and draw considerable attention in pharmacological research due to their anti-inflammatory, anti-malarial, anti-hepatitis, and anti-cancer activities (Khan et al, 2012; Lee et al, 2003; Tan et al, 1998). Jaceosidin (Figure 1), isolated from Artemesia argyi, has been reported to have promising biological effects, including anti-tumorigenic, antioxidant, and anti-inflammatory activities (Khan et al., 2012; Kim et al, 2008; Min et al, 2009). Jaceosidin has anti-proliferative effect and induces apoptosis in various cancer cells such as endometrial cancer cells (Lee et al, 2013), ovarian cancer cells (Lv et al, 2008), and glioblastoma U87 cells You must attribute the work in the manner specified by the author or licensor

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Results

Conclusion