Tryptophan supplementation and pH adjustment for optimizing the sporulation of Coniothyrium minitans

Abstract

To determine the effect of tryptophan and pH on sporulation of Coniothyrium minitans, the fungus was cultivated using a two-stage, agar plate method in which addition of tryptophan and pH were controlled at the sporulation stage. The spore yield was enhanced by 4 times with 0.1 g tryptophan/l addition after 72 h. The optimal pH values were 4 for mycelia growth and 5.8-6 for sporulation. Mycelia grown at pH 6 had a higher productivity of spore production than did those grown at pH 4.