Abstract
A simple and rapid screening method for microorganisms with phospholipase A1, A2 and C activities using agar plate and gas chromatography (GC) method was successfully carried out. In agar plate method, soy bean lecithin and taurocholic acid were used as carbon source and emulsifier, respectively. In this agar plate method, microorganisms with phospholipase A1 and A2 or C activity produce a halo around the colony and two kinds(A's and C) of microorganisms are clearly distinguished by turbidity of the halo. Microorganisms with phospholipase A1 and A2 activity is simply distinguished by GC using a synthetic phospholipid containing different fatty acid at sn-1 and sn-2 position.
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