Tryptophan phosphorescence study of the internal dynamics of human erythrocyte membrane proteins upon spectrin modification

Abstract

Room-temperature tryptophan phosphorescence has been used analyze the slow (millisecond) internal dynamics of proteins in isolated native human erythrocyte membranes, after removal of 95% of spectrin, and after thermal denaturation of spectrin or medium acidification to pH 6.0–4.0, as well as the internal dynamics of spectrin extracted from the membrane in solution. The integral membrane proteins prove to differ sharply from spectrin in their structural and dynamic state. The millisecond movements of structural elements in integral proteins are considerably hindered as compared with spectrin. Removal of the bulk of spectrin from membranes leads to amplification of slow fluctuations in the structure of integral proteins. This suggests involvement of spectrin in the control of the structural and dynamic state of the erythrocyte membrane proteins. The acidification of the medium to pH 6.0–4.0 decreases the internal dynamics of native membrane proteins, which is explained by the pH-induced aggregation of spectrin. After thermal denaturation of spectrin, there is no pH-induced increase in the rigidity of the structure of membrane proteins.