Trypanocidals effectivity against some isolates of Trypanosoma evansi propagated in mice

Didik Tulus Subekti,F Amaliah,B Santosa,Sulinawati Sulinawati,H Susiani,I Yuniarto

doi:10.14334/jitv.v20i4.1246

Abstract

Surra is one of infectious diseases in various types of animals caused by blood parasites called Trypanosoma evansi. It is fatally occurred, especially in horse, buffalo and camel. Surra may be controlled by effectively trypanocidals treatment based on the results of its sensitivity test. Therefore, it is necessary to study the effectiveness of various trypanocidal against some T.evansi isolates originating from several regions in Indonesia with surra case to determine its suitability and efficacy. The test was carried out by pre-test - post-test. Mice were infected by several T.evansi isolates from various infected areas. Their parasitaemia were observed. After reaching peak of parasitemia, the mice were treated by trypanocidal with different doses. Parasitaemia alteration was observed for one month. Observation results showed that all isolates had different sensitivity to the trypanocidals, so that trypanocidals application tended to specific location. Generally, suramin and melarsomine dihydrochloride were the most effective trypanocidal for some Indonesian isolates. In contrast, isometamidium chloride was not recommended to be used for surra control in Indonesia. Key Words: Trypanosoma evansi , Trypanocidal, Parasitemia, Surra

Highlights

Surra is one of infectious diseases in various species of animals caused by blood parasites called Trypanosoma evansi (T. evansi)
On the Subekti et al Trypanocidals effectivity against some isolates of Trypanosoma evansi propagated in mice contrary, Nzelibe et al (2013) tested trypanocidal activity of Azadirachta indica seed extract (NSE) and leaves of Tridax procumbens (TP) which showed a success as trypanocidal without relapse when it was combined
Kabi et al (2009) reported that Swiss Webster mice that have been infected with T.evansi isolates from Uganda and treated with diminazene diaceturate were all dead on 18th day postinfection. These results correspond with the results of Zhang et al that proves the failure of diminazene diaceturate to kill T.evansi isolate from China, the Philippines and Ethiopia, both in vitro and in vivo (Kabi et al 2009). These results indicate that the T.evansi isolates from Uganda used by Kabi et al (2009) had higher resistance than Saudi Arabia isolate used by Mohammed (2008), Pakistan isolate used by Akbar et al (1998), and six isolates from Indonesia were used in this study

Summary

INTRODUCTION

Surra is one of infectious diseases in various species of animals caused by blood parasites called Trypanosoma evansi (T. evansi). On the Subekti et al Trypanocidals effectivity against some isolates of Trypanosoma evansi propagated in mice contrary, Nzelibe et al (2013) tested trypanocidal activity of Azadirachta indica seed extract (NSE) and leaves of Tridax procumbens (TP) which showed a success as trypanocidal without relapse when it was combined. Those extracts were not available commercially, and economically those extractions were impossible to be applied to cow, buffalo, and horse. It is important to investigate the effectiveness of various trypanocidal against some T.evansi isolates originating from Indonesia, especially from the region of cases or outbreaks of Surra

MATERIAL AND METHOD

AND DISCUSSION

Findings

CONCLUSION