Abstract

Both free and esterified sterols and triterpenes of Spanish Moss ( Tillandsia usneoides L.; Bromeliaceae0 from Florida were examined. Besides the eight sterols and triterpenes previously reported by others as present in Spanish Moss from Mexico, cycloeucalenol, 24-methylenecycloartanol and two unidentified triterpenes of the cycloartane group were detected by GLC and GLC-Mass Spectroscopy. Campesterol and stigmasterol were detected by these techniques and cholesterol was detected by GLC. Attempts to determine the specific nature of the esters of both triterpenes and desmethyl sterols were thwarted by the extreme complexity of the ester mixture. However, saturated fatty acids from C 12 to C 30 were detected in the sterol and triterpene ester fraction by GLC, with palmitic acid (25%) stearic acids (20%) predominating as the fatty acid moieties.

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