Abstract
New minor triterpene glycoside, cucumarioside D (1) identified earlier as a candidate to new glycoside using LC-ESI QTOF-MS has been isolated from the sea cucumber Eupentacta fraudatrix as individual substance. The structure of the glycoside was elucidated using 2D NMR spectroscopy and MS. The glycoside has non-sulfated pentasaccharide carbohydrate chain branched by the second sugar (quinovose) and having 3-O-methylglucose as a terminal monosacharide residue that is an unique structural feature of sea cucumber triterpene glycosides from this species. A moderate cytotoxic activity of the glycoside 1 against the ascyte form of Echlich carcinoma cells and hemolytic activity has been found.
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