Tripterygium Wilfordii Polyglycosides (TP) Ameliorate Alcoholic Kidney Injury in Rats by Regulating Protein Expression Associated with the Nuclear Factor Erythroid 2-Related Factor 2 Signaling Pathway

Abstract

Objective: To explore the mechanisms of protection of tripterygium wilfordii polyglycosides (TP) against alcoholic kidney injury by regulating Nrf2 signaling pathway in rats. Materials and Methods: Dividing rats into Control, Model, LD, MD and HD groups. The kidney was weighed to calculate kidney index. The morphology of the kidney was observed by HE staining. Nrf2, p-Nrf2 and HO-1 in kidney were detected by immunohistochemistry. Measuring Scr by Jaffe’s method and BUN by diacetyl-oxime method. The renal SOD and MDA were detected by colorimetry. The renal ROS was detected by fluorescence spectrometry. Results: Compared with Control, histopathological changes were observed in Model group, The kidney index, Scr, BUN, renal MDA and ROS concentrations increased significantly (P <0.001). Renal SOD activity, expression of p-Nrf2 and HO-1, p-Nrf2/Nrf2 decreased significantly (P < 0.001). With TP supplement, compared with Model, histopathological was improved, The kidney index, Scr, BUN, renal MDA and ROS concentrations decreased significantly (P <0.05, respectively); Renal SOD activity, p-Nrf2 and HO-1, p-Nrf2/Nrf2 increased significantly (P <0.05, respectively) in TP treated groups (LD, MD and HD). Conclusion: TP can prevent or reduce oxidative stress and attenuate alcoholic kidney injury via regulating Nrf2 signaling pathway in vivo.