Abstract
Tripartite motif containing 25 (TRIM25) is a member of TRIM proteins and functions as an E3 (ubiquitin ligase). It has been found to act as an oncogene in gastric cancer cells and is abnormally expressed in cancers in female reproductive system. Here, we investigated the function of TRIM25 in colorectal cancer. TRIM25 was found to be significantly up-regulated in colorectal cancer tissues and cancer cell lines through real-time PCR assay. Colorectal cancer cells (CRCs) overexpressing TRIM25 exhibited a two-fold higher proliferation and migration rate compared with their parental lines in vitro. Moreover, TRIM25 also promoted tumor progression in vivo. Further study indicated that TRIM25 worked through positively regulating transforming growth factor β (TGF-β) signaling pathway to regulate the proliferation and invasion of CRCs. In summary, our results indicate that TRIM25 also acts as an oncogene in colorectal cancer and it functions through TGF-β signaling pathway. Thus, TRIM25 represents potential targets for the treatment of colorectal cancer.
Highlights
Colorectal cancer is the third most common cancer and the third leading cause of cancer deaths in men and women in the United States [1]
The results showed that Tripartite motif containing 25 (TRIM25) was significantly overexpressed in colorectal cancer tissues, compared with the corresponding adjacent tissues (Figure 1A)
cancer cell (CRC) lines (HT29 and HCT116) have a significantly elevated expression of TRIM25 compared with the colonic epithelial cell line FHC (Figure 1B)
Summary
Colorectal cancer is the third most common cancer and the third leading cause of cancer deaths in men and women in the United States [1]. Colorectal cancer shows significant stage dependence of the 5-year overall survival rate [2]. Colorectal cancer is undoubtedly a major health threat to world population. The majority of colorectal cancer metastasis is to the liver, and surgical resection is the most effective therapy for liver metastases of colorectal cancer. Metastatic recurrence following curative surgery is the leading cause of mortality [4,5]. It is important to understand the biological mechanisms underlying colorectal cancer progression and identify the factors that contribute to the metastases of colorectal cancer
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