Abstract
Trimethylamine (TMA) biosensor was constructed by immobilizing flavin-containing monooxygenase type-3 (FMO3), as one of drug metabolizing enzymes in human liver, onto a sensitive area of a dissolved oxygen electrode.The FMO3 immobilized sensor with flow injection analysis (FIA) was calibrated against trimethylamine solutions (putrefactive substance of fish) from 1.0 to 50.0mmol/l, with good reproducibility in the repeatedly measurements (coefficient of variation: 4.39%, n = 5).By applying the TMA sensor for a fish-freshness analysis with extractions of horse-mackerel, the sensor output increased with the holding time of fish samples at 25°C because of their decomposition. The TMA sensor with FMO3 would be convenient device for evaluating fish freshness.
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