Abstract
Proteins belonging to the TRIM family have been implicated in a variety of cellular processes such as apoptosis, differentiation, neurogenesis, muscular physiology and innate immune responses. Trim69, previously identified as a novel gene cloned from a human testis cDNA library, has a homologous gene in zebrafish and this study focused on investigating the function of trim69 in zebrafish neurogenesis. Trim69 was found to be expressed in zebrafish embryo brain at the early stages. Knockdown of trim69 led to deformed brain development, obvious signs of apoptosis present in the head, and decreased expression of neuronal differentiation and stem cell markers. This phenotype was rescued upon co-injection of human mRNA together along with the trim69 knockdown. Results of this study also showed an interaction between TRIM69 and c-Jun in human cells, and upon TRIM69 knock down c-Jun expression subsequently increased, whereas the over-expression of TRIM69 led to the down-regulation of c-Jun. Additionally, knockdown both c-Jun and trim69 can rescue the deformed brain, evident cellular apoptosis in the head and decreased expression of neuronal differentiation and stem cell markers. Overall, our results support a role for trim69 in the development of the zebrafish brain through ap-1 pathway.
Highlights
The TRIM protein family (The tripartite motif protein family, TRIM), called the RBCC family, consists of proteins containing a tripartite motif composed of a RING domain, one or two B‐box domains and a Coiled‐coil region[8]
We focused on investigating the role of trim[69] in zebrafish neurogenesis and the molecular mechanism through which it carries out its function
In order to determine its role in zebrafish development, we examined the loss of function phenotype utilizing trim69-MO antisense oligonucleotides
Summary
The TRIM protein family (The tripartite motif protein family, TRIM), called the RBCC family, consists of proteins containing a tripartite motif composed of a RING domain, one or two B‐box domains and a Coiled‐coil region[8]. Proteins belonging to this family have been implicated in a variety of cellular processes; including apoptosis, cell cycle regulation, neurogenesis, muscular physiology and innate immune responses[9,10]. We focused on investigating the role of trim[69] in zebrafish neurogenesis and the molecular mechanism through which it carries out its function
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