Triladyl® improves the cryopreserved quality and in vivo fertilization potential of Beetal buck (Capra hircus) spermatozoa

Abstract

This study compared differential concentrations of chicken egg yolk (v/v; T1, 10%; T2, 15%; and T3, 20%) in commercial extender Triladyl® with tris-citric acid based extender (v/v; chicken egg yolk: 20%; C, control) for improving the in vitro quality and in vivo fertilization potential of Beetal buck (Capra hircus) spermatozoa at post–thawing. The mean values of progressive motility (PM, %), rapid velocity (RV, %), average path velocity (VAP, μm sec−1), straight line velocity (VSL, μm sec−1), curvilinear velocity (VCL, μm sec−1), supra-vital plasma membrane integrity (SV−PMI, %), mitochondrial transmembrane potential (MMP, %), and viable with intact acrosome (V−IACR, %) were significantly higher(except significantly lower medium velocity; MV, %)in T1 and T2with than control at all stages. Mean values of straightness (STR – VSL: VAP %) and linearity (LIN – VSL: VCL %) were significantly higher in T1 and T2 than control at PD. In vitro longevity, from 0 to 3 h incubation (37 °C), showed significantly low PM (T1) and RV (T1 and T2) percentage loss than control at PT. A significantly positive Pearson’s correlation was noticed between CASA and SV−PMI, MMP, V−IACR, and DNA integrity (%). Significantly higher in vivo fertilization rates (%) were recorded in T1 (73.53) and T2 (74.19) compared to the C (46.88) and T3 (48.28), respectively. In conclusion, addition of even chicken egg yolk at 10% (v/v) in Triladyl® improves the cryopreserved in vitro and in vivo quality of Beetal buck spermatozoa as compared to tris-citric acid extender with 20% (v/v) egg yolk.