Triggered antioxidant defense mechanism in maize grown in soil with accumulation of Cu and Zn due to intensive application of pig slurry

Abstract

The present study investigated changes in both the growth parameters and the enzymatic and non-enzymatic antioxidant systems of maize (Zea may L.) plants grown in Typic Hapludalf soil containing an accumulation of Cu and Zn. This accumulation developed because the soil received nineteen applications of pig slurry in no-tillage system over seven years. In this study, the maize plants were grown for fifteen and 25 days after emergence (DAE) in pots containing undisturbed and disturbed soil samples collected from a field experiment that received the rates 0, 20, 40 and 80m3ha−1 of pig slurry, which totalized the amount of 0, 380, 760 and 1520m3ha−1 of pig slurry in seven years, respectively, and phosphorus (P)+potassium (K) treatment (in disturbed soil samples). The maize plants grown in the undisturbed soil samples with an accumulation of Cu and Zn did not indicate an apparent decrease in growth. However, when compared to the treatment with PK fertilization, the maize plants grown in the disturbed soil with pig slurry treatments indicated higher lipid peroxidation and a number of senescent leaves, as well as a significant decrease in plant height. Additionally, when compared to the PK treatment, the leaf superoxide dismutase and ascorbate peroxidase activities decreased and increased, respectively, with the addition of pig slurry treatments in the disturbed soil at 25 DAE. In general, when compared to the treatments with 20m3ha−1 of pig slurry and PK at fifteen and 25 DAE, the leaf ascorbic acid and non-protein thiol groups concentrations decreased with the addition of 40 and 80m3ha−1 of pig slurry. This result suggests that the excess of Cu and Zn in the pig slurry significantly changed the antioxidant system of the maize plants.