Treatment with hCG 4 or 6 days after TAI to improve pregnancy outcomes in repeat-breeding dairy cows

Abstract

A study was conducted to evaluate the effect of human chorionic gonadotropin (hCG) administration 4 or 6 days after timed AI (TAI) on P4 concentration and pregnancy outcomes in repeat breeding dairy cows. All cows were treated by Cosynch protocol before being assigned into 3 groups: CON (n=139): did not receive hormonal treatments; D4 (n=136): received 1500IU hCG 4 days post TAI; and D6 (n=131): received 1500IU hCG 6 days post TAI. Plasma P4 concentration was evaluated on Day of hCG and 12 days post TAI. Pregnancies per AI (P/AI) on Day 45 were greater for D6 (38.9%) than CON (30.9%) groups. Pregnancy losses (PL) were lower for D6 (15.0%) and D4 (26.7%) than CON (37.7%) groups. Treatment by season revealed a greater P/AI and lower PL for D6 (30.3% and 18.2%) and D4 (33.3% and 31.0%) than CON (19.2% and 58.3%) groups during summer, while P/AI was greater for D6 (57.1%) than D4 (30.6%) and CON (37.9%) groups during winter. Treatment by parity indicated a greater P/AI for D4 (46.4%) and D6 (31.6%) than CON (15.8%) groups within primiparous cows. Pregnancy losses were lower for D6 (15.1%) than CON (36.5%) groups within multiparous cows while D4 tended to be lower (13.3%) than CON (50.0%) groups. Plasma P4 concentration on Day 12 post TAI was higher for treated than for CON groups. Applying hCG 6 days after TAI was beneficial in improving P/AI either in summer or winter as a result of reducing PL and increasing P4 concentration.