Abstract
Lactating Holstein cows (n=141) were synchronized to receive their first timed artificial insemination (TAI). Blood and milk samples were collected 25 and 32d after TAI, and pregnancy status was determined 32d after TAI using transrectal ultrasonography. Cows diagnosed pregnant with singletons (n=48) continued the experiment in which blood and milk samples were collected and pregnancy status was assessed weekly using transrectal ultrasonography from 39 to 102d after TAI. Plasma and milk samples were assayed for pregnancy-associated glycoprotein (PAG) levels using commercial ELISA kits. Compared to ultrasonography, accuracy was 92% for the plasma PAG ELISA test and 89% for the milk PAG ELISA test 32d after TAI. Plasma and milk PAG levels for pregnant cows increased from 25d to an early peak 32d after TAI. Plasma and milk PAG levels then decreased from 32d after TAI to a nadir from 53 to 60d after TAI for the plasma PAG assay and from 46 to 67d after TAI for the milk PAG assay followed by an increase from 74 to 102d after TAI. Overall, plasma PAG levels were approximately 2-fold greater compared with milk PAG levels, and primiparous cows had greater PAG levels in plasma and milk compared with multiparous cows. The incidence of pregnancy loss from 32 to 102d after TAI based on ultrasonography was 13% for cows diagnosed with singleton pregnancies, and plasma and milk PAG levels decreased to nonpregnant levels within 7 to 14d after pregnancy loss. Both plasma and milk PAG levels were negatively correlated with milk production for both primiparous and multiparous cows. We conclude that stage of gestation, parity, pregnancy loss, and milk production were associated with plasma and milk PAG levels after TAI similarly. Based on plasma and milk PAG profiles, the optimal time to conduct a first pregnancy diagnosis is around 32d after AI, coinciding with an early peak in PAG levels. Because of the occurrence of pregnancy loss, all pregnant cows should be retested 74d after AI or later when plasma and milk PAG levels in pregnant cows have rebounded from their nadir.
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