Abstract
Soybean peroxidase (SBP) catalyzes the oxidation and polymerization of aromatic compounds in the presence of hydrogen peroxide. The polymerized products precipitate from solution, providing an alternative to conventional treatment methods. Studies were conducted to characterize the use of polyethylene glycol (PEG) as an additive to increase the active life of the enzyme. The effectiveness of PEG increased with its molecular weight, with maximum protection accomplished with PEG of molecular weight 35,000. Linear relationships were found between the quantity of phenol to be treated (1.0–10 mM) and the optimum doses of SBP and PEG required for greater than 95% removal. Observations suggest that it is the interaction between the PEG and the polymeric products that results in the protection of SBP. Following treatment, approximately 25% of the optimum PEG dose remained in the supernatant.
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