Treatment forms of house dust mites allergens intended for allergen-specific immunological therapy

Abstract

BACKGROUND: The growth of allergic diseases dictates the necessity to develop new forms of therapeutic allergens since therapy with native water-salt extracts of allergens is unsafe and convenient. The risk of systemic side effects during allergen-specific immunotherapy sets the task for researchers to create modern therapeutic forms of allergens with high immunogenic and low allergenic activity. Our laboratory has been developing preparations for the diagnosis and treatment of house dust mites allergies for many years.
 AIMS: To create modern therapeutic forms of allergen preparations from house dust mites of the genus Dermatophagoides that are intended for allergen-specific immunotherapy
 MATERIALS AND METHODS: Physicochemical and immunobiological methods, such as electrophoresis in polyacrylamide gel, micropoint immunoblot, solid-phase enzyme-linked immunosorbent assay, and the reaction of binding inhibition of allergen-specific immunoglobulin E in the sera of patients were used to study the obtained preparations.
 RESULTS: A technology has been developed to obtain a granular dosage form of a mixed allergen from Dermatophagoides pteronyssinus and Dermatophagoides farinae mites for sublingual use. This treatment form has successfully passed preclinical tests, has a pronounced immunogenic activity, and reduced allergenicity, and is convenient for pediatric practice. Allergoids, which are chemically modified preparations, are obtained to reduce allergenicity and increase therapeutic allergen immunogenicity. A succinylated monomeric house dust mites allergoid Dermatophagoides pteronyssinus was created and studied with the National Research Center, Institute of Immunology Federal Medical-Biological Agency of Russia. The study of the immunobiological properties of the obtained preparation showed that the monomeric allergoid has increased immunogenic and decreased allergenic activity contrary to the native water-salt extract.
 CONCLUSIONS: The created forms of mite allergens can be used to treat patients who are sensitized to house dust mites of the genus Dermatophagoides after clinical trials.