Treatment Activity of Ho(III)-Based Coordination Polymer on Liver Cancer by the Inhibition of Vascular Endothelial Growth Factor Signaling Pathway Activity

Abstract

A new 0D dinuclear coordination polymer [Ho2(L)6(phen)2] (1) was hydrothermally synthesized based on HoCl3·6H2O, organic ligand HL = 3-hydroxybenzoic acid, and the auxiliary ligand phen = 1,10-phenanthroline (L− is the fully deprotonated organic ligand), and full characterization of the structure was performed via the X-ray single-crystal diffraction data. Once this newly synthesized novel compound was achieved, the way it acted inside the liver cancer was examined, and the corresponding mechanism was determined. First, the Cell Counting Kit-8 (CCK-8) method was conducted to analyze the compound activity after the treatment of liver cancer cells. In addition, real-time reverse-transcription polymerase chain reaction (RT-PCR) method was used to examine the in-cell vascular endothelial growth factor (VEGF) signaling pathway activity. The molecular docking simulation showed that the carboxyl and phenol groups contained active binding receptor sites, indicating that Ho complex has excellent biological activity.