Abstract
The analogy that electrons flowing in wires is like water flowing through a tube can be remarkably effective for teaching and learning about fluid flow in LC systems. In this installment, we apply the concepts developed in last month’s installment by demonstrating how they can be used to help troubleshoot problems in LC involving pressure and flow. We also introduce several free tools that can be used to calculate pressure drops in different elements of LC systems, including connecting capillaries and packed columns. Knowing what pressure drops to expect for system components under different chromatographic conditions is very valuable in many troubleshooting situations.
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