Abstract

The effects of temperature, permeability and the presence of a residual oil phase on the transport of the bacterium Geobacillus sp. were investigated experimentally in sandpack cores. A lower reduction in permeability and a higher effluent bacterial concentration were obtained in the higher permeability sandpacks, or at a higher temperature. The bacterial transport could be enhanced by the pulse injection, but reduced with the presence of residual oil. When the injected cell concentration is relatively high (>108 cells/mL), log-jam effect or pore bridging may be the primary mechanism, and the surface adhesion may act as the secondary mechanism of bacteria retention and permeability reduction.

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