Abstract
Segments of hamster small intestine have been used to study the interaction of 5-thio- d-glucose with the d-glucose transport system. By all current criteria, 5-thio- d-glucose is a substrate for the system. We have examined accumulation against a concentration gradient, substrate inhibition, phlorizin sensitivity, Na + dependence, energy dependence and counterflow. The estimated K m value for 5-thio- d-glucose is of the order of 2.8 mM. Two interesting differences between d-glucose and 5-thio- d-glucose have been found. 5-Thio- d-glucose is less effective than d-glucose in inhibiting the specific binding of d-[ 3H]glucose to brush borders. 5-Thio- d-glucose is also a poor substrate for hexokinase. The effect of the sulphur substitution on possible ring conformation of the sugar is discussed with respect to the specificity of the transport system.
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