Abstract
2-Deoxy- d-glucose transport by Rhodotorula glutinis is an active process. The intracellular concentration of free deoxyglucose after 15 min incubation of Rhodotorula cells with this sugar was 230 times the extracellular concentration. Although cell extracts at this time contained more 2-deoxy- d-glucose 6-phosphate than deoxyglucose, pulse-labelling experiments demonstrated that deoxyglucose is transported as the free sugar and subsequently phosphorylated. After transport, Rhodotorula cells metabolize deoxyglucose. The major metabolites during 30–90 min incubations were determined to be 2-deoxy- d-glucose 6-phosphate, 2-deoxy- d-glucitol, 2-deoxy- d-gluconate and 2,2′-dideoxy-α,α′-trehalose. Rhodotorula glutinis also degrades deoxyglucose to CO 2. The concentrations of intermediates in this pathway were too low to detect and resolve in extracts of control cells. In 2,4-dinitrophenol-poisoned cells, however, it appears that deoxyglucose degradation is restricted largely to loss of C-1 as CO 2 and it was possible to identify 1-deoxy- d-ribulose 5-phosphate as an intermediate presumably arising from metabolism of deoxyglucose by the oxidative portion of the hexose monophosphate pathway.
Published Version
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