Transport and fate of viruses in sediment and stormwater from a Managed Aquifer Recharge site

Abstract

Enteric viruses are one of the major concerns in water reclamation and reuse at Managed Aquifer Recharge (MAR) sites. In this study, the transport and fate of bacteriophages MS2, PRD1, and ΦX174 were studied in sediment and stormwater (SW) collected from a MAR site in Parafield, Australia. Column experiments were conducted using SW, stormwater in equilibrium with the aquifer sediment (EQ-SW), and two pore-water velocities (1 and 5 m day−1) to encompass expected behavior at the MAR site. The aquifer sediment removed >92.3% of these viruses under all of the considered MAR conditions. However, much greater virus removal (4.6 logs) occurred at the lower pore-water velocity and in EQ-SW that had a higher ionic strength and Ca2+ concentration. Virus removal was greatest for MS2, followed by PRD1, and then ΦX174 for a given physicochemical condition. The vast majority of the attached viruses were irreversibly attached or inactivated on the solid phase, and injection of Milli-Q water or beef extract at pH = 10 only mobilized a small fraction of attached viruses (<0.64%). Virus breakthrough curves (BTCs) were successfully simulated using an advective–dispersive model that accounted for rates of attachment (katt), detachment (kdet), irreversible attachment or solid phase inactivation (μs), and blocking. Existing MAR guidelines only consider the removal of viruses via liquid phase inactivation (μl). However, our results indicated that katt > μs > kdet > μl, and katt was several orders of magnitude greater than μl. Therefore, current microbial risk assessment methods in the MAR guideline may be overly conservative in some instances. Interestingly, virus BTCs exhibited blocking behavior and the calculated solid surface area that contributed to the attachment was very small. Additional research is therefore warranted to study the potential influence of blocking on virus transport and potential implications for MAR guidelines.