Abstract
The primary cilium has recently become the focus of intensive investigations into understanding the physical structure and processes of eukaryotic cells. This paper describes two tilt-series image datasets, acquired by transmission electron microscopy, of in situ chick-embryo sternal-cartilage primary cilia. These data have been released under an open-access licence, and are well suited to tomographic reconstruction and modelling of the cilium.
Highlights
First identified in 1894 by Zimmermann [1], the primary cilium is a non-motile singular cytoplasmic organelle found in virtually all vertebrate cells
This paper presents the transmission electron microscopy (TEM) tilt-series dataset used for the original 3D reconstruction [4,20] of primary cilia
Samples were fixed in 2.5% glutaraldehyde with 1600 ppm ruthenium hexaamine tri-chloride (RHT) in 0.1 M sodium cacodylate buffer for 16 h at 37 °C, and post-fixed in 1% osmium tetroxide (OsO4) with 1600 ppm RHT at 4 °C for 4 h, followed by dehydration through a progressive series of graded ethanol concentrations
Summary
First identified in 1894 by Zimmermann [1], the primary cilium is a non-motile singular cytoplasmic organelle found in virtually all vertebrate cells. Electron tomography, or imaging biological structures using transmission electron microscopy (TEM) for 3D reconstruction, is widely used [3,19] This allows for a greater detailed nanometer-scale spatial characterisation and modelling of structures with no loss of information due to sectioning [2,4,20,21]. These techniques involve imaging a sample through a series of tilt-angles in order to acquire data for later reconstruction into 3D volumes [22]. The data, as presented, are in a form that can be reconstructed in 3D using tools, such as IMOD [22]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
