Abstract
Fatty acid synthetase messenger RNA was obtained from rat liver polysomal RNA and then injected into Xenopus laevis oocytes. The radioactive fatty acid synthetase protein synthesized in the oocytes was identified by immunoprecipitation with anti-fatty acid synthetase antibody and the immunoprecipitate was then characterized by electrophoresis on sodium dodecyl sulfate-polyacrylamide gel. Co-migration of authentic fatty acid synthetase and the labeled product synthesized in oocytes was observed. Based on sucrose density gradient analysis, the rat liver fatty acid synthetase mRNA has a sedimentation coefficient of approximately 33 S, which agrees with the predicted minimum size necessary to code for the fatty acid synthetase protein. In addition, this mRNA was partially purified with oligo(dT)-cellulose, which indicates that it has a polyadenylate region. The relative in vivo rate of synthesis of fatty acid synthetase and the level of fatty acid synthetase mRNA in liver were also determined during the course of dietary induction of this enzyme. The results indicate that the dietary-induced increase in the level of fatty acid synthetase is probably due to an increased level of the fatty acid synthetase mRNA.
Highlights
From the Lipid Metabolism Laboratory, William S
The results indicate that the dietary-induced increase in the level of fatty acid synthetase is probably due to an increased level of the fatty acid synthetase mRNA
The fatty acid synthetase synthesized in the oocytes was identified by specific immunoprecipitation with antiserum to native fatty acid synthetase and by the co-migration of synthesized “H-labeled protein and authentic fatty acid synthetase protein on SDS
Summary
Of the Fatty Acid (Received for publication, March 3, 1977, and in revised form, September 30, 1977). From the Lipid Metabolism Laboratory, William S. Middleton Memorial Veterans Hospital, and the Department of Physiological Chemistry, University of Wisconsin, Wisconsin 53706. Administration Madison, Fatty acid synthetase messenger RNA was obtained from rat liver polysomal
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