Abstract
Streaming currents were measured in silica slit-like nanochannels with integrated electrodes using a pulsed flow of deionized water. The analysis of the transient streaming current measurements in nanochannels shows that streaming currents are proportional to the surface charge density σ and that the effective reduction of nanochannel height by immobilized biomolecules needs to be taken into account. The σ of silica nanochannels was measured after performing sequential chemical surface modification steps. The nanochannel surface was first altered with positively charged polylysine and then with non-charged polyethylene glycol showing that nanochannels can sense the charge and size of surface-immobilized molecules.
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