Abstract
Transient state isoelectric focusing (TRANSIF) is a kinetic method which offers quantitative information about relevant parameters pertaining both to methodological aspects and to the physical characterization of amphoteric molecules. TRANSIF data are obtained with an improved scanning isoelectric focusing assembly coupled to an on-line digital data acquisition and processing system which is used to continuously record changes in peak position (x), peak area, segmental pH gradient (Δ(pH)Δx), and isoelectric point (pI) of proteins during focusing. This improved apparatus has made it feasible to follow the temporal stability of an isoelectric focusing system in a quantitative fashion.
Published Version
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