Abstract
Objective: Autologous adult stem cell therapy for cardiovascular disease has shown minimal or no benefit in clinical trials. This may in part be explained by aging-related alteration of secretory function and loss of replicative capacity of autologous cell preparations. Previously we have shown that functional and replicative capacity of human aortic endothelial cells can be increased by retroviral overexpression of telomerase. However, there is a concern for immortalization and insertional mutagenesis with this approach. More recently, we have developed methods for transient expression of human telomerase reverse transcriptase (TERT). We hypothesize that this method will increase telomere length (TL) sufficiently to enhance the regenerative capacity of adult stem cells. In the current study we assessed the effects of this approach on adipose-derived stromal vascular fraction (SVF).
Published Version
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