Abstract
The localization of phenolic compounds in tubers of transgenic potatoes overexpressing enzymes of the flavonoid synthesis pathway, i.e. chalcone synthase (CHS), chalcone isomerase (CHI) and dihydroflavonol dehydrogenase (DFR), was examined by fluorescence microscopy. The study revealed the presence of flavonoids (anthocyanins) in cell vacuoles, mainly in the peridermis. A relatively higher concentration of anthocyanin compounds was observed in the tissue of transgenic potato tubers, revealing the expression of chalcone synthase (CHS). The increased content of flavonoids in transgenic potato tubers may affect their agrotechnical traits and may increase the nutritive value of the tubers.
Highlights
Chalcone synthase (CHS), chalcone isomerase (CHI) and dihydroflavonol dehydrogenase (DFR) play key roles in the synthesis of flavonoids
"Free-hand" sections of potato tuber peridermis of control and transformed plants were studied under bright field and fluorescence microscopy
Microscopic examination of the tissues of transgenic potato tubers overexpressing the enzymes chalcone synthase (CHS), CHI and DFR showed the presence of phenolics in the vacuoles of cells located in the peridermal celi layer
Summary
Chalcone synthase (CHS), chalcone isomerase (CHI) and dihydroflavonol dehydrogenase (DFR) play key roles in the synthesis of flavonoids. (2003) employing liąuid chromatography/mass spectrometry revealed elevated concentrations of pelargonine and petunidine in transgenic potato tubers of the plants, indicating the overexpression of CHI and DFR. The aim of the study was to determine, using microscopic methods, the localization and degree of concentration of cellular structures containing polyphenolic compounds in the tissue of transgenic plants overexpressing enzymes of the flavonoid synthesis pathway as well as eventually, to visualize the differences between the tissues of transgenic potato tubers of the 3 studied lines and of the traditional line of potatoes. The study materiał included the potato tubers of transgenic Solarium tuberosum L. cv Desiree.
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