Abstract
There are two isozymes of angiotensin-converting enzyme (ACE), one produced by somatic tissues and a smaller protein synthesized by developing spermatozoa (testis ACE). To investigate the molecular control of testis ACE, we generated mice transgenic for a construct containing a putative testis-specific ACE promoter linked to the Escherichia coli reporter gene encoding beta-galactosidase. The transgenic mice express beta-galactosidase protein and RNA only within the testis. Histochemical analysis of the transgenic mice shows co-localization of beta-galactosidase protein and endogenous ACE within elongating spermatozoa. These studies demonstrate that transcription of testis ACE is controlled by a strong intragenic testis-specific promoter that is contained within a 698-base pair fragment immediately upstream from the transcription start site of testis ACE. Characterization of the testis ACE promoter may provide insights into the molecular mechanisms controlling cell stage-specific gene expression in the male germ line.
Highlights
There are two isozymes of angiotensin-converting enzyme (ACE), one producedby somatic tissues and a smaller protein synthesized by developing spermatozoa
Later stages of spermiogenesis (Fig. 4A).These are the same cell stages that contain testis ACE as detected by reaction with an anti-ACE antisera (Fig. 4B).thetestis-specific ACE promoter region appears to maintain its in uiuopattern of cell type-specific transcription even when linked to the/3galactosidase reporter gene
Analysis of cDNA encoding the somatic form of the enzyme has demonstrated that somatic ACE is a single polypeptide chain composed of twohomologous domains, each about half the size of the parent molecule and each containing a potential catalytic site (14, 15)
Summary
There are two isozymes of angiotensin-converting enzyme (ACE), one producedby somatic tissues and a smaller protein synthesized by developing spermatozoa (testis ACE). To investigate the molecular control of testis ACE, we generated mice transgenic for a construct containing a putative testis-specific ACE promoterlinked to the Escherichiacoli reportergene encoding @-galactosidase.
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