Abstract
ABSTRACT Electroporation was used to introduce hygromycin phosphotransferase gene into creeping bentgrass (Agrostis palustris Huds.) protoplasts isolated from embryogenic suspension cells. After the subsequent selections with 50 mg L1 hygromycin, several plantlets were regenerated from hygromycin resistant calli. PCR and Southern hybridization analysis of randomly chosen plants indicated that the transgene was integrated into their genomes and no false positives were found. The results suggested that hygromycin phosphotransferase gene was effective selectable marker gene for the selection of transformed cells. Overall frequency for hygromycin resistant colony formation in this report was 1-3 × 10−6 based on the number of treated protoplasts.
Published Version
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