Transformation of human cells by polyoma and Rous sarcoma viruses mediated by inactivated Sendai virus.

Abstract

AbstractTransformation of embryonic human fibroblasts was obtained by treatment with inactivated Sendai virus and polyoma virus. Transformed cells differed morphologically from normal cells and formed a stable cell line (P‐2). A stable transformed cell line (23) was also formed after absorption of Rous sarcoma virus (Schmidt‐Ruppin strain) and inactivated Sendai virus on the embryonic human fibroblasts.Cell line P‐2 and cell line 23 contained a human chromosome set, although some karyological abnormalities were apparent.The cell lines P‐2 and 23 showed some characteristics of malignant cells growing in vitro (ability to multiply when suspended in semi‐solid medium and to form tumours in the cheek pouches and brains of golden hamsters). The potent transforming factor (s) was/were synthesized by these cells. The transforming factors had some properties identical to those of polyoma or Rous viruses and caused rapid transformation of embryonic mouse and human cells growing in vitro.Foci of epithelioid cells appeared in human embryonic fibroblasts after the absorption of human adenovirus type 12 and inactivated Sendai virus.