Abstract
Numerous organisms were examined to find a fungal system capable of catalyzing aflatoxin transformations. Some fungi, particularly Dactylium dendroides, Absidia repens, and Mucor griseo-cyanus, transform about 50–60% of aflatoxin B1 in shake cultures to a new fluorescent-blue compound (R0), with a lower Rf (0.57) than B1 (0.69) on silica gel thin-layer chromatographic plates (acetone:CHCl3, 20:80). In a separate assay, washed fungal mycelium suspended in 0.02 M PO4 buffer catalyzed a 25–30% conversion in 20 h. The new fluorescent compound was separated from the unchanged B1 by column chromatography on silica gel G with washed CHCl3 containing 6% acetone and 0.75% ETOH as solvent. The ultraviolet spectrum showed maxima of 325, 261, and 254 mμ. Its infrared spectrum, showing a shift in absorption peaks at 1760 and 1685 cm−1, indicated a change in the functional carbonyls of B1; in addition, a broad band at 3400 cm−1 indicated a hydroxylated compound.
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