Transformasi Genetik Tembakau dengan Gen Cold Shock Protein melalui Perantara Agrobacterium tumefaciens

Abstract

<p>Cold<br />shock protein (Csp) essential for organisms to survive in<br />abiotic stress condition. CspB gene has been fused to<br />ubiquitin promoter in the T-DNA region of pCambia 1300int,<br />and introduced into Agrobacterium tumefaciens LBA4404.<br />This research had an objective to transform genetically<br />Nicotiana tabacum cv. Samsun by CspB gene under the<br />control of Ubiquitin promoter and NOS terminator mediated<br />by A. tumefaciens. Leaf discs were co-cultivated with A.<br />tumefaciens LBA 4404. Based on the number of hygromycinresistant<br />calli, the efficiency of transformation was 57.5%. In<br />the selective medium containing 50 μg/l hygromycin, the<br />efficiency of regeneration of transgenic shoots was 82.6%.<br />Based on PCR analysis using primers corresponding to<br />ubiquitin promoter and CspB gene, 18 putative tobacco<br />transgenic containing CspB gene under the control of<br />ubiquitin promoter.</p>