Abstract
Currently development of new marker types has shifted from anonymous DNA fragments to gene-based markers. Simple Sequence Repeats (SSRs) are useful DNA markers in plant genetic research including in peanut. However, de novo development of SSRs is expensive and time consuming. Gene-based DNA markers are transferable among related species owing to the conserved nature of genes. In this study transferability of sorghum EST-SSR (SbEST-SSR) markers to peanut was prospected. A set of 411 SbEST-SSR primer pairs were used to amplify peanut genomic DNA extracted from cultivated peanut where 39% of them successfully amplified. A comparison of amplification patterns between sorghum and peanut showed similar banding pattern with majority of transferable SbEST-SSRs. Among these transferable SSR markers, 14% have detected polymorphism among 4 resistant and 4 susceptible peanut lines for rust and late leaf spot diseases. These transferable markers will benefit peanut genome research by not only providing additional DNA markers for population genetic analyses, but also allowing comparative mapping to be possible between peanut and sorghum—a possible monocot-dicot comparison.
Highlights
Peanut (Arachis hypogaea L.) is an important oilseed crop and it has acquired prominence because of its economic importance as well as its nutritional value
Simple Sequence Repeats (SSRs) or microsatellites have become one of the most widely preferred molecular marker systems for genetic analysis for their advantages compared to other molecular markers: high reproducibility, high polymorphism, being multi-allelic, co-dominant, higher relative abundance and extensive genome coverage are some of the advantages envisaged with SSRs [5]
We focused on analyzing the utility of EST-SSR markers from sorghum to peanut experimentally
Summary
Peanut (Arachis hypogaea L.) is an important oilseed crop and it has acquired prominence because of its economic importance as well as its nutritional value. It is the third major oilseed crop in the world only to soybean and cotton. Molecular markers have become an important tool in crop breeding programs for dissecting loci controlling complex traits: genetic diversity among accession and evolutionary conservation studies can be done. Application of molecular markers in peanut crop improvement has been relatively lagging behind owing to limited knowledge of genome and seldom molecular variations revealed by RFLP [1], RAPD [2] and isozyme markers systems [3]. Previous studies in peanut have shown that SSR markers could detect more polymorphism than other molecular markers like RFLPs [6], AFLPs [7] and RAPDs [8,9]
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