Abstract
High oleic trait, resistance to rust and late leaf spot (LLS) are important breeding objectives in groundnut. Rust and LLS cause significant economic loss, and high oleic trait is an industry preferred trait that enhances economic returns. This study reports marker-assisted selection to introgress high oleic content, resistance to LLS and rust into Kadiri 6 (K 6), a popular cultivar. The alleles for target traits were selected using linked allele-specific, simple sequence repeats and single nucleotide polymorphic markers. The F1s (384), intercrossed F1s (441), BC1F1s (380), BC1F2s (195), and BC1F3s (343) were genotyped to obtain desired allelic combination. Sixteen plants were identified with homozygous high oleic, LLS and rust resistance alleles in BC1F2, which were advanced to BC1F3 and evaluated for disease resistance, yield governing and nutritional quality traits. Phenotyping with Near-Infrared Reflectance Spectroscopy identified three lines (BC1F3-76, BC1F3-278, and BC1F3-296) with >80% oleic acid. The identified lines exhibit high levels of resistance to LLS and rust diseases (score of 3.0–4.0) with preferred pod and kernel features. The selected lines are under yield testing trials in multi-locations for release and commercialization. The lines reported here demonstrated combining high oleic trait with resistance to LLS and rust diseases.
Highlights
Groundnut or peanut (Arachis hypogaea L.) has diversified uses; it is used for food, feed, fodder, and industrial purposes (Sahdev, 2015)
Two independent crosses were attempted with Kadiri 6 (K 6) as a pistillate parent and ICGV 15033, and ICGV 13193 (LLS and rust-resistant line) as pollen parents to generate F1s during rainy 2016
The polymerase chain reaction (PCR) products amplified using simple sequence repeat (SSR) marker GM2301 were separated using capillary electrophoresis followed by electropherogram assay (Figure 3B) which could differentiate recipient and donor type alleles with 260 bp and 235 bp, respectively, and true hybrids showed both the alleles
Summary
Groundnut or peanut (Arachis hypogaea L.) has diversified uses; it is used for food, feed, fodder, and industrial purposes (Sahdev, 2015). It is cultivated across 118 countries covering 27.94 million hectares (Mha) area, with an annual production of 47.09 million tons (MT) of pods. India ranks first with an area of 5.30 Mha, and second in production with 9.17 MT of pods (FAOSTAT, 2017). The global vegetable oil consumption is expected to double by 2040 including groundnut oil (FAOSTAT, 2015). The groundnut kernel fat consists of monounsaturated fatty acid, oleic acid (36–81.3%), and polyunsaturated fatty acid, linoleic acid (3.9–40.2%) (Janila et al, 2016b).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
