Replicated phenotypic data and KASP genotypic data used to combine high oleic acid trait and resistance to late leaf spot and rust diseases in groundnut (Arachis hypogaea L.)

Abstract

This data corresponds to a study that reports marker-assisted selection to introgress high oleic content, resistance to LLS and rust into Kadiri 6 (K6), a popular cultivar. The diagnostic SNPs were utilized for genotyping the breeding population. The SNP markers used in the study consisted of one SNP for ahFAD2B positioned at +442 bp in the ORF on the 9th chromosome of the B genome associated with a high oleic trait. The validated five diagnostic SNPs present on A03 associated with LLS and rust resistance were used. The two leaf discs of 5 mm each were collected from each plant for genotyping using Kompetitive allele-specific PCR (KASPTM) assay. A total of 195 BC1F2 seedlings were raised and screened for zygosity using SNP markers. The assay revealed 16 plants with homozygous alleles for LLS, rust, and ahFAD2B allele which were advanced through selfing to generate BC1F3 seeds. The allele call of six SNPs is presented in this data.