Abstract
The results of a study to identify the transfer RNA's for every amino acid in human tissues are reported. The isoaccepting species of the tRNA of human lymphoblasts (normal and leukemic) were fractionated by reverse-phase partition column chromatography. In every case, comparisons were made between the aminoacyl-tRNA profile of normal and leukemic lymphoblasts by co-chromatography using 3H for one label and 14C for another and the homologous amino acid-activating enzyme. Differences between the profiles of normal and leukemic cells were re-evaluated by reversal of the labeled amino acid and by acylation with the heterologous enzyme. The results indicate that (1) there are at least 56 species of tRNA fractionated from both the normal and the leukemic cells; (2) for most cases the profiles of the leukemic cells were very similar if not identical to the profiles of normal cells; (3) small but reproducible differences were found for leucyl-, seryl-, threonyl- and prolyl-tRNA; (4) the most pronounced differences were with tyrosyl-tRNA and glutaminyl-tRNA. The difference in the glutaminyl-tRNA was an extra isoaccepting species found only when normal lymphoblast enzyme preparation was used. Therefore, in this case the difference was due to the enzyme preparation rather than the tRNA itself.
Published Version
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