Abstract
Transepithelial fluctuation analysis (noise analysis) provides valuable information about the density and single-channel properties of ion channels in intact epithelia. Here we investigate cystic fibrosis transmembrane conductance regulator (CFTR)-dependent chloride (Cl −) secretion in T84 human colonic epithelia by inducing noise using the diarylsulfonylurea DASU-01, a low-affinity open-channel blocker of CFTR. Our data indicate that the apical membrane of maximally stimulated T84 epithelia has a very high Cl − conductance generated by ∼7000 active CFTR channels per cell with open probability ( P o) of ∼0.4 and single-channel amplitude ( i) of ∼0.1 pA. Similar experiments might provide important information about how drugs regulate CFTR in intact epithelia.
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